SECTION 1 THE BASICS
What is a haemocytometer --> FLASH animation

What does it do? --> FLASH animation
The main features --> FLASH animation
Preparing the cells --> FLASH animation
Viewing the cells under a microscope --> FLASH animation
Quiz

SECTION 2 COUNTING THE CELLS

TITLE: PREPARING THE CELLS AND THE CHAMBER

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Make your cell suspension before you start and follow these steps to prepare your haemocytometer.

  1. Polish the mirrored surface with lens paper to clean it.
  2. Polish and clean the cover slip.
  3. Place the cover slip on the chamber surface. Place it on by sliding it across the surface until you can see Newton’s rings or the colours of the spectrum, because this will indicate that the cover slip is firmly attached by friction to the slide.
  4. Using a pasteur or other type of pipette, introduce your suspension under the cover slip or into the “V” shaped grove if your slide has one.
  5. The area under the cover slip will fill by capillary action. You should pipette enough fluid so that the counting surfaces are covered and some residue leaks out into the trough.
  6. The prepared haemocytometer is then placed on the microscope stage ready for counting.

 

 

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