VAL Resources

The lab skills resources are all listed in the table below. Many are designed using various versions of Macromedia / Adobe FLASH. Most have also been published as video on YouTube as part of another open education project called Biology Courses. You can visit the YouTube channel by searching for biologycourses.

TITLE
Description
Content Producer

Cell Culture

GIVE US FEEDBACK
Basic tissue culture techniques for mammalian cells



1) Resuscitating cells
, 2) Subculturing (splitting cells) and 3) Cryogenic storage

Technician team

Student notes and quizzes



PDF and Word documents containing notes and quizzes

Technician team

Haematology

GIVE US FEEDBACK
How to prepare a blood smear



Video from the Leicester Royal Infirmary pathology department
on preparing a blood smear

LRI biomedical scientists

The full blood count

Extensive narrated slide presentation from Queen's College Belfast that is simply too fabulous not to share

Professor McMullin

Automated large-scale blood analysis

Series of 10 videos from the Leicester Royal Infirmary


LRI biomedical scientists

Sickle cell resources
Sickle cell open education project website (SCOOTER) SCOOTER team and collaborators

Haemocytometry

GIVE US FEEDBACK

Introduction to the haemocytometer

Animations and video clips introducing the haemocytometer which is commonly used in the lab for counting cells.

Ruta Furmonavicience, Viv Rolfe

Counting cells

Animations explaining further principles of counting cells and using the Neubar grid

Ruta Furmonavicience, Viv Rolfe

Performing calculations

Examples of how to perform calculations of cell concentration and opportunities to try for yourself!

Ruta Furmonavicience, Viv Rolfe


Extras

1) Volume Conversion How to convert units of volume
2) Table Calculating Grid Volumes How to calculate the volume of liquid under the grid.

Ruta Furmonavicience, Viv Rolfe

Histology and the Haematoxylin and Eosin (H&E) Stain

GIVE US FEEDBACK
Historical background to the stain
Background to the history of the Haematoxylin and Eosin stain and the histochemical method. Mike Ball
H&E steps

1) Step-by-step guide to the H&E stain
and
2) clearing and mounting the slide

Mike Ball
Stain chemistry
Basic principles of stain chemistry Viv Rolfe
Stain recipes
Assorted goodies - a classroom stain manual and PowerPoint presentations Viv Rolfe

Microbiology

GIVE US FEEDBACK

Basic incubation experiment

Short videos explaining how to set up basic aerobic and anaerobic microbial incubations

Marilena Ioannou

Agar plate

How to streak an agar plate Malgorzata Rekas

Using API strips

1) Video of how to set up an API strip -
2) API biochemistry -
3) Reading the strip results

Marilena and Malgorzata

Using the portable autoclave

1) Parts of the autoclave and 2)Using a portable autoclave to sterilise laboratory equipment

Technical team


Gram stain technique

1) Preparing for the Gram stain for viewing bacteria and 2) Extra Gram stain resource

Marilena and Malgorzata

Microscopy

GIVE US FEEDBACK
Parts of the microscope

Animation introducing parts of the microscope and a quiz to test you self.


Vivien Rolfe

Setting up the light microscope
Video clips demonstrating how to set up a light microscope.
Basic steps to prepare the microscope.

Simon Oldroyd

Setting up the light microscope
Video demonstrating how to maintain a light microscope.
Maintenance
Technical Team
Check list
Check list for setting up the microscope.

Simon Oldroyd

Pipettes

GIVE US FEEDBACK
How to use a pipette

Graham Basten

How to use a pipette

Graham Basten

How to use a pipette

Graham Basten

Serial Dilutions

GIVE US FEEDBACK
Introduction to the serial dilution

Five animations demonstrating how to perform dilutions and serial dilutions

Graham Basten

Spectrophotometry

GIVE US FEEDBACK

Introduction to the spectrophotometer

Video introducing the spectrophotometer

Ruta Furmonaviciene, Viv Rolfe

How the spec works

Series of six animations

Ruta Furmonaviciene, Viv Rolfe

The Bradford Assay
Animation and video of the Bradford
protein assay
Ruta Furmonaviciene, Viv Rolfe

Standard Curves

GIVE US FEEDBACK
Drawing standard curves

1) Animation describing the standard curve and 2) how to interpret the results

Graham Basten

Standard curves in Excel

1) Using Excel (or other spreadsheet) to generate a standard curve and 2) Interactive tutorial

Viv Rolfe

Generating the linear equation

1) Using Excel to produce a linear equation and 2) Interactive tutorial

Viv Rolfe

Calculations
Using Excel to calculate the value of your unknown sample

Viv Rolfe


Should you find any blurps, bloops, cordwangles, red wine stains or gremlins within these resources, please Tweet @vivienrolfe.


Funding acknowledgements
Home Page - Contact - Terms & Accessibility - User Guide - Site Map