PERFORMING AND INTERPRETING STANDARD CURVES

Preparing solutions for a standard curve --> FLASH animation

Interpreting the resulting graph --> FLASH animation

Using Excel to create the graph --> Adobe Captivate tutorial

Have a go yourself --> Captivate interactive tutorial

Using Excel to produce a linear equation --> Captivate tutorial

Have a go yourself --> Captivate interactive tutorial

Using Excel to make final calcuations --> Captivate tutorial

TITLE: PREPARING SOLUTIONS FOR THE STANDARD CURVE

If we now look at sequential dilutions, these are really important if we want to make accurate calibration curves. So for example if we need a 1 in 2, 1 in 5, 1 in 10, 1 in 20, 1 in 50, 1 in 100, 1 in 500 and a 1 in 1000, at first this could seem quite daunting. All we need to do is initially make a 1 in 2, 1 in 5 and 1 in 10, this is because the rest are simply serial 1 in 10 dilutions of these.

Lets look at this in stages. Stage 1. 1 part A and 1 part B gives a 1 in 2 dilution (C2). 1 part A and 4 parts B gives a 1 in 5 (C5). 1 part A and 9 parts B gives a 1 in 10 (C10).

Stage 2. Take 1 part of C2 and 9 parts B gives us 1 in 20 (C20). 1 part of C 5 and 9 parts of B gives us 1 in 50 (C50). 1 part of C10 and 9 parts B gives us 1 in 100 (C100). Dilute C50 and C100 10 fold to give us 1 in 500 (C500) and 1 in 1000 (C1000). We make dilutions in this way for reasons of accuracy.